Using the matched data between infrared images of the geostationary meteorological satellite Himawari-8 and the product 2AGPROFGMI of Global Precipitation Measurement (GPM), the matched infrared brightness temperature (BT), brightness temperature difference (BTD) and surface precipitation are connected by pixel to pixel. Further more, because of the advantage of the more channels of geostationary meteorological satellite, two-dimensional and three-dimensional lookup tables of rain rate (RR) are established with the matched infrared brightness temperature, brightness temperature difference and surface precipitation. The lookup tables which can identify the rain rate of different grades are found by the retrieval tests. The three-dimensional lookup tables (BT10.4, BTD12.4-10.4, BTD6.2-7.3) show higher probability of detection (POD=0.8817) and lower false alarm rate (FAR=0.4042) when detecting the rain, so it can identify the areas of rainfall.

Two Anaerobic Filter (AF)-Biological Aerated Filter (BAF) systems, one for bioaugmented system and another for control system, were set-up to treat coking wastewater, and the influence of the introduced nitrogenous heterocyclic compounds on the reactor performance was investigated. The results showed that the COD removal efficiency of AF in both bioaugmented and control system was 35% while the BOD₅/COD increased from 0.33 to 0.59 after the treatment of AF in both systems. When 100 mg/L carbazole was introduced to the influent, the COD removal efficiency of the bioaugmented AF remained 35% and BOD₅/COD of the bioaugmented AF effluent decreased to 0.53, while the COD removal efficiency of the control AF declined to 23% and BOD₅/COD of the control AF effluent dropped to 0.45. When 100 mg/L quinoline and 50 mg/L pyridine were introduced to the influent simultaneously, the COD removal efficiency of the bioaugmented AF decreased to 27% and BOD₅/COD of the bioaugmented AF effluent decreased to 0.48, while the COD removal efficiency of the control AF declined to 12% and BOD₅/COD of the control AF effluent decreased to 0.38. Moreover, the removal efficiencies of the introduced carbazole, quinolone and pyridine were 83%, 91% and 88% respectively after bioaugmented AF treatment, while the removal efficiencies of the introduced carbazole, quinolone and pyridine were 57%, 66% and 55% respectively after the control AF treatment. This indicated that the bioaugmented system was more effective to resist the impact of high concentration nitrogenous heterocyclic compounds. The GC-MS analysis of the bioangmented AF effluent indicated that the introduction of nitrogenous heterocyclic compounds led to an increment of the alkane and benzoic esters in the AF effluent. This study revealed the impact of the high concentration of organic compounds on A/O system for treating coking wastewater.

Radio frequency identification (RFID) suffers extremely limited chip area and energy resource. A novel elliptic curve cryptographic (ECC) processer based on generalized binary Hessian curve (GBHC) is designed and implemented. The authors employ Montgomery Ladder scalar-multiplication algorithm and optimized w-coordinate method for accelerating the computing timing, and well-design circular shift register (CSR) architecture and clock gating technology for reducing the consumption of area and energy. The results show that the proposed processer has fast computing speed, minimal chip area and ultra-low energy consumption, and is capable to resist some types of side channel attack (SCA) such as simple power analysis (SPA).

The authors studied the DPA attack method and circuit level protection technology, and introduced a security circuit WDDL on FPGA and a new symmetrical routing technology. A 4-bit WDDL adder on FPGA (field programmable gate array) platform was implemented and the power consumption of the circuit was analyzed. The results show that power consumption of WDDL decreases obviously than that of the traditional circuit and WDDL circuit can reduce the correlation of power consumption and data effectively. WDDL is proved to have better anti DPA (differential power analysis) attack ability at the cost of chip size.

To study the vulnerability of Advanced Encryption Standard (AES) against electromagnetic side channel attacks, based on the method of correlation electromagnetic analysis (CEMA) attack, the authors built a platform to acquire EM emanation and process data, then performed a near-field CEMA attack against an FPGA implementation of AES-128. The results indicate that the platform is able to acquire the EM emanation of the encryption chip, and can retrieve all the 16 bytes of the 10th roundkey of AES. After the optimization of processing data, the efficiency of CEMA is highly enhanced, namely the data needed to exploit the correct roundkey is greatly reduced.

The authors discuss the performance and area of different large-scale Booth multipliers with high radices used in Montgomery algorithm using secondary encoded scheme. The modular multiplication is implemented with SMIC 0.13μm technology at the frequency of 160 MHz and 125 MHz respectively based on the 128-bit multiplier and 256-bit multiplier with Booth 64, 128 and 256 encoding. Experiment result shows that the multiplier with Booth 64, 128 and 256 can achieve the same timing performance, while the area rises as radix rises due to the complexity in pre-computation and partial product generation.

The bacterioplankton community structure in Wujin River was investigated by terminal restriction fragment length polymorphism (T-RFLP) technique in combination with 16S rRNA clone library analysis. The T-RFLP fingerprints analysis shows high diversity and low evenness among the bacterioplankton community. A 16S rRNA gene clone library was also constructed. The results based on Ribosomal Database Project Ⅱ show that there were relatively abundant bacterial species in Wujin River and the predominant bacteria belonged to the genera of Proteobacteria. The BLAST results indicate the proportion of clones with middle or high similarity to known sequences in GenBank was high, while many unknown microorganisms were also present. Moreover, the possible environmental links of known genera were also discussed.

MTS1 is a tumor suppressor gene with potential clinical application. It was mutated in many primary tumors and cell lines. For its special properties, adenovirus attracts more and more attentions in tumor gene therapy. Carcino-embryonic antigen (CEA) promoter can convey cell type specific expression of genes. MTS1 gene driven by CEA promoter and polyadenylation signal were inserted into the E1 region of adenovirus by means of homologous recombination. The influence of recombinant adenovirus on the growth of breast cancer cell line MCF7 was studied. Results show that the recombinant adenovirus can inhibit the growth of MCF7.

A phage display 15mer random peptide library expressed on the N-terminal of the filamentous phage M13 minor coat protein pⅢ was constructed using the phagmid pCANTAB5G8. The capacity of library is 1.36×10⁸, calculated from the transformation efficiency. Human recombinant Intercellular Adhesion Molecule 1 was immobilized on the ELISA plates. After 4 rounds affinity panning and ELISA analysis, 5 positive phage clones that can bind to ICAM-1 specifically were selected. These positive clones were sequenced and the amino acid sequence of 15mer were deduced.The Ka of one positive clone was 7.87×10⁷, measured by ELISA.

The murine phage antibody library was amplified and then panned by human chorionic gonadotropin (hCG) for four rounds. The last round enriched phage clones were used to reinfect E coli TG1 and 15 separate colonies were assayed by ELISA. The recombinant phages which were rescued from colony 12# showed the highest affinity to immobilized hCG. The Scfv gene was inserted into the reconstructed pET-21a(+) and expressed in E coli BL21(DE3). The dot blot hybridization verified that the ScFv was secreted to the medium.

Based on the structure and function analysis of hirudin, a potent thrombin inhibitor, and some platelet aggregation inhibitors, which contain the recognition sequence Arg-Gly-Asp(RGD)as their functional motif, two chimeric antithrombotic molecules were designed by introducing RGD sequence to hirudin C terminus. These chimera genes were constructed by PCR and inserted into the expression vector pET-21a(+), the constructs were confirmed by restriction enzyme digestion and DNA sequence analysis. These recombinant plasmids were transformed into E.coli strain BL21(DE3), and gene expression was induced by IPTG. The target proteins were directed into the periplasmic space by the staphylococcal protein A(SPA)signal sequence preceding the RGD-hirudin gene. Using ion exchange chromatography and gel filtration chromatography, the chimera proteins were purified, and both of them showed a single band in Tricine-SDS-PAGE.The results of activity analysis suggested that these two chimera proteins not only have antithrombin activities, but gain platelet aggregation inhibitory activities as well.

Fibrin-specific antibodies were isolated from a phage-displayed single-chainantibody(ScAb) library using affinity selection or panning. DNA shuffling was introduced to realign the specific antibody genes in order to mimic the antibody maturation in vivo. After cloning of shuffling products, a secondary library was constructed, from which the specific antibody against fibrin with higher activity was selected through panning and screening. The fibrin-specific antibody gene and uPA functional domain gene fragment were joined together, then inserted into expression plasmid pET-21a. The ScAb/uPA fusion protein was expressed with the induction of IPTG. In substrate S₂₄₄₄ assay, the uPA activity of the chimera was about 3.1*10³ IU/mg periplasmic protein of host E.coli. It also kept up the affinity to fibrin.

A murine phage antibody library containing 1.7×10⁸ independent clones was constructed and affinity panning of anti-N-peptide antibody fragments was carried out. First, mRNA was purified from the total RNA extracted from fresh spleens of nonimmunized mice of Kunming, then the cDNA library was achieved via reverse transcription PCR. Gene fragments encoding V_H and V_L were amplified and assembled into a single gene using a DNA linker encoding a polypeptide of 15 amino acid residues (Gly₄Ser)₃ through primary PCR. And finally the recombinant DNA fragments were cloned into the phagemid pCANTAB 5E vector and introduced into E.coli TG1 by means of electroporation. The phagemid particles displaying functional ScFvs were rescued by reinfection of helper phage M13KO7, thus a murine antibody library was obtained. One N-peptide-binding ScFv clone was selected from the phage antibody library using affinity panning. The target antigen, N-peptide was biotinylated using photobiotin first. Then the biotin-labelled N-peptide interacted with the phage antibody library. The streptavidin paramagnetic beads were added subsequently to bind the biotinylated N-peptide-specific phage particle complex. Washing, elution and reinfection were ensued sequentially. After 4 rounds panning, one clone was verified to show higher affinity to N-peptide by dot blot.

Adenosine triphosphate (ATP) is a kind of purinergic neurotransmitter. By means of intraperitoneal injection, intrapreoptical microinjection and Neuronal study of preoptic area of hypothalamic (POAH) tissue slice, effects of ATP on thermoregulation in the rat were investigated. The results are as follows. (1) there occurred an appreciable hypothermia followed by intraperitoneal injection of ATP with dose-dependent effect. Pindolol, a noradrenaline-β receptor antagonist, abolished the effect and suggested that the Tb fall was presumptively mediated by sympathetic nervous system; (2) Intrapreoptical administration of ATP with lower concentration decreased the body temperature, but the effect could be dramatically eliminated by reactive blue 2, a nonselective P₂-purinoceptor antagonist, hinting the likelihood that purinoceptors play an important role in thermoregulation; The higher doses, however, elicited the body temperature rise, which might be attributed to the chelating effect of ATP on free extracellular calcium; (3) The effects of ATP on the warm-sensitive neurons in POAH slices gave further evidence for the result showed in vivo, the higher and lower concentrations of ATP inhibited and facilitated the spontaneous firing of warm-sensitive neurons respectively, which suggested that ATP involved in thermoregulation by direct affecting the warm-sensitive neuron activity in POAH.

An improved, sensitive and precise capillary gas chromatographic method with electron capture detector has been developed for the determination of isosorbide-5-mononitrate in human plasma. 2, 4-Dinatrotoluene is used as the internal standard. The plasma is extracted with ether in sodium carbonate solution. The residue was reconstructed with methanol. The linear correlation coefficient between concentration and height ratio is better than 0.999. The recovery was found to range from 92% to 104% for three concentration levels of samples with a limit of determination 0.000005g/L and with the relative standard deviation of 8% within-day.

According to the amino acid sequence of hirudin HV-2, six pieces of polynucleotides were designed and chemically synthesized. The whole hirudin gene was constructed with these polynucleotides by recursive PCR. Then the synthetic gene was inserted into the secretary expression vector, pIN-ompA-HI. The recombinant plasmids were introduced into competent cells of E.coli strain DH 5a. Its DNA sequence was determined, proved to be the same as designed. The hirudin gene was expressed in E.coli. The recombinant product was secreted into the periplasm and medium, and antithrombin activity could be detected. The expression level was 19.8% of bacteria total proteins. The product was purified by salting out, gel filtration chromatography, and ion exchange chromatography. SDS-PAGE of the product showed a single band of 12k Da(as a dimer)and mass spectrometry showed the molecular weight of 6893.1 Da. It is suggested that the recombinant hirudin has been processed correctly during the secretion.

The theory of resonant interaction of planetary waves in a two layer model is extended from three-wave system to five-wave system. The evolution equations governing the amplitudes of the waves are derived and solved analytically. It is found that the solutions can be given in terms of Jacobi elliptic functions.